abstract：:The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescenc...

journal_title：Applied biochemistry and biotechnology

authors： Xu J,Zhu W,Guo Y,Jiang C,Hussain N,Meng L,Lu S

更新日期：2017-02-01 00:00:00

abstract：:Seven white rot fungal species were tested for growth as mycelia using swine wastewater (SW), an agro-waste with tremendous environmental footprint, as the sole nutrient source. The SW contained high concentrations of carbon and nitrogen components, which could support nutritional requirements for mycelial growth. Out...

journal_title：Applied biochemistry and biotechnology

authors： Lee J,Shin SG,Ahn J,Han G,Hwang K,Kim W,Hwang S

更新日期：2017-02-01 00:00:00

abstract：:The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

journal_title：Applied biochemistry and biotechnology

authors： Sharma S,Meena LS

更新日期：2017-02-01 00:00:00

abstract：:Based on the principle of self-coagulation of microorganisms, the flocculant-producing denitrifying bacterial TN-14 sludge was added to the continuous-flow reactor for treating domestic sewage. The bacterial TN-14 sludge acted as the main seed sludge to promote the rapid formation of aerobic granular sludge. The sludg...

journal_title：Applied biochemistry and biotechnology

authors： Xin X,Lu H,Yao L,Leng L,Guan L

更新日期：2017-01-01 00:00:00

abstract：:In rapidly growing developing countries, waste disposal is a major challenge. Current waste disposal methods (e.g., landfills and sewage treatment) incur costs and often are not employed; thus, wastes accumulate in the environment. To address this challenge, it is advantageous to create economic incentives to collect ...

journal_title：Applied biochemistry and biotechnology

authors： Lonkar S,Fu Z,Wales M,Holtzapple M

更新日期：2017-01-01 00:00:00

abstract：:In this study, starches obtained from wheat, potato, and corn were used to synthesize cationic starches (CS), and the flocculation efficiency of these materials was tested with Chlorella pyrenoidosa and Botryococcus braunii cultures under different conditions. Our results indicated that these three CS had differing de...

journal_title：Applied biochemistry and biotechnology

authors： Peng C,Li S,Zheng J,Huang S,Li D

更新日期：2017-01-01 00:00:00

abstract：:The effects of amino acid composition and peptide molecular mass on ACE-inhibitory and antioxidant activities of protein fragments obtained from tomato waste fermented using Bacillus subtilis were evaluated. The addition of B. subtilis increased the relative amounts of aromatic and positively-charged amino acids which...

journal_title：Applied biochemistry and biotechnology

authors： Moayedi A,Mora L,Aristoy MC,Hashemi M,Safari M,Toldrá F

更新日期：2017-01-01 00:00:00

abstract：:Euryale ferox Salisbury is an important aquatic food plant cultivated largely in eastern India. E. ferox is a monotypic genus, and breeding programmes have mostly relied on the variability present in the primary gene pool. Knowledge of the genetic structure of the population is limited, and there are very few reports ...

journal_title：Applied biochemistry and biotechnology

authors： Kumar H,Priya P,Singh N,Kumar M,Choudhary BK,Kumar L,Singh IS,Kumar N

更新日期：2016-12-01 00:00:00

abstract：:Glutathione (γ-glutamyl-L-cysteinylglycine, GSH) is a pharmaceutical compound often used in food additives and the cosmetics industry. GSH can be produced biologically from L-glutamic acid, L-cysteine, and glycine through an enzymatic process traditionally involving two sequential adenosine triphosphate (ATP)-dependen...

journal_title：Applied biochemistry and biotechnology

authors： Jiang Y,Tao R,Shen Z,Sun L,Zhu F,Yang S

更新日期：2016-12-01 00:00:00

abstract：:Chicken feathers are available in large quantities around the world causing environmental challenges. The feathers are composed of keratin that is a recalcitrant protein and is hard to degrade. In this work, chicken feathers were aerobically pretreated for 2-8 days at total solid concentrations of 5, 10, and 20 % by B...

journal_title：Applied biochemistry and biotechnology

authors： Patinvoh RJ,Feuk-Lagerstedt E,Lundin M,Sárvári Horváth I,Taherzadeh MJ

更新日期：2016-12-01 00:00:00

abstract：:Titanium-dioxide nanoparticles (TNP) are used in various consumable goods. Evidence has demonstrated the cytotoxicity of TNPs, but exact mechanism is yet to be elucidated. The present study has been aimed at finding out the mechanism of TNP-induced toxicity in biological system. Different doses of anatase-TNPs adminis...

journal_title：Applied biochemistry and biotechnology

authors： Kumar S,Meena R,Paulraj R

更新日期：2016-12-01 00:00:00

abstract：:Recently, poly(3-hydroxybutyrate) (PHB) has been found in a few thermophilic strains where several advantages can be gained from running fermentation at high temperatures. Caldimonas manganoxidans, a thermophilic gram-negative bacterium, was investigated for the feasibility as a PHB-producing strain. It is suggested t...

journal_title：Applied biochemistry and biotechnology

authors： Hsiao LJ,Lin JH,Sankatumvong P,Wu TM,Li SY

更新日期：2016-11-01 00:00:00

abstract：:One novel microbial esterase PHE21 was cloned from the genome of Pseudomonas oryzihabitans HUP022 identified from the deep sea of the Western Pacific. PHE21 was heterologously expressed and functionally characterized to be a robust esterase which behaved high resistance to various metal ions, organic solvents, surfact...

journal_title：Applied biochemistry and biotechnology

authors： Wang Y,Zhang Y,Hu Y

更新日期：2016-11-01 00:00:00

abstract：:Endophytes from medicinal plants represent a potential source of bioactive compounds. During the present investigation, fungal endophytes were isolated from turmeric (Curcuma longa), an important medicinal plant. A total of 207 endophytic fungal isolates were obtained from the rhizome of C. longa L. They were grouped ...

journal_title：Applied biochemistry and biotechnology

authors： Gupta S,Kaul S,Singh B,Vishwakarma RA,Dhar MK

更新日期：2016-11-01 00:00:00

abstract：:Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research. Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transfo...

journal_title：Applied biochemistry and biotechnology

authors： Dang X,Shan Z,Chen H

更新日期：2016-11-01 00:00:00

abstract：:The RNA-guided endonuclease clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) derived from CRISPR systems is a simple and efficient genome-editing technology applied to various cell types and organisms. So far, the extensive approach to detect the cleavage activity of custo...

journal_title：Applied biochemistry and biotechnology

authors： Yang Y,Liu S,Cheng Y,Nie L,Lv C,Wang G,Zhang Y,Hao L

更新日期：2016-10-01 00:00:00

abstract：:Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on sub...

journal_title：Applied biochemistry and biotechnology

authors： Rao K,Chodisetti B,Gandi S,Giri A,Kishor PB

更新日期：2016-10-01 00:00:00

abstract：:Chiral 2-chloropropanoic acids and their ester derivatives are crucial intermediates in the synthesis of many chemicals, especially herbicides. The enzymatic synthesis of chiral 2-chloropropanoic acids and their ester derivatives by esterases was not easily achieved, because the structural difference between the two e...

journal_title：Applied biochemistry and biotechnology

authors： Cao Y,Deng D,Sun A,Zhang Y,Hu Y

更新日期：2016-09-01 00:00:00

abstract：:Protein-protein interactions (PPIs) play pivotal roles in most of the biological processes. PPI dysfunctions are therefore associated with disease situations. Mutations often lead to PPI dysfunctions, but there are certain other types of mutations which do not cause any appreciable abnormalities. This second type of m...

journal_title：Applied biochemistry and biotechnology

authors： Ali A,Biswas R,Bhattacharjee S,Nath P,Pan S,Bagchi A

更新日期：2016-09-01 00:00:00

abstract：:This investigation utilized sugarcane bagasse aqueous extract (SBAE), a nontoxic, cost-effective medium to boost triacylglycerol (TAG) accumulation in novel fresh water microalgal isolate Scenedesmus sp. IITRIND2. Maximum lipid productivity of 112 ± 5.2 mg/L/day was recorded in microalgae grown in SBAE compared to mod...

journal_title：Applied biochemistry and biotechnology

authors： Arora N,Patel A,Pruthi PA,Pruthi V

更新日期：2016-09-01 00:00:00

abstract：:Streptomyces coelicolor A3(2) sco5931 gene was predicted to encode a putative xylanase A, a 477 amino acid protein belonging to glycoside hydrolase family 10. The entire sco5931 coding region was cloned and overexpressed in Streptomyces lividans TK24. Mature SCO5931 protein comprising 436 amino acids (47 kDa) was puri...

journal_title：Applied biochemistry and biotechnology

authors： Enkhbaatar B,Lee CR,Hong YS,Hong SK

更新日期：2016-09-01 00:00:00

abstract：:This study aimed to investigate fragments derived from human and bovine lactoferrins for ability to inhibit nuclear translocation of HIV-1 integrase. It was shown that human lactoferricin, human lactoferrin 1-11, and bovine lactoferrampin reduced nuclear distribution of HIV-1 integrase. Bovine lactoferrampin could inh...

journal_title：Applied biochemistry and biotechnology

authors： Wang WY,Wong JH,Ip DT,Wan DC,Cheung RC,Ng TB

更新日期：2016-08-01 00:00:00

abstract：:This work aimed to characterize two native microalgal strains newly isolated from South Mediterranean areas and identified as Chlorella sorokiniana ES3 and Neochloris sp. AM2. The growth properties and biochemical composition of these microalgae were evaluated in different culture media (Algal, BG-11, f/2, and Conway)...

journal_title：Applied biochemistry and biotechnology

authors： Jazzar S,Berrejeb N,Messaoud C,Marzouki MN,Smaali I

更新日期：2016-08-01 00:00:00

abstract：:Tomato pomace is an abundant lignocellulosic waste stream from industrial tomato processing and therefore a potential feedstock for production of renewable biofuels. However, little research has been conducted to determine if pretreatment can enhance release of fermentable sugars from tomato pomace. Ionic liquids (ILs...

journal_title：Applied biochemistry and biotechnology

authors： Allison BJ,Cádiz JC,Karuna N,Jeoh T,Simmons CW

更新日期：2016-08-01 00:00:00

abstract：:Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Ma J,Zhang Y,Zhang M,Wu M,Dai Z,Jiang M

更新日期：2016-07-01 00:00:00

abstract：:To date, two types of glycerol dehydratases have been reported: coenzyme B12-dependent and coenzyme B12-independent glycerol dehydratases. The three-dimensional structure of the former is a dimer of αβγ heterotrimer, while that of the latter is a homodimer. Their mechanisms of reaction are typically enzymatic radical ...

journal_title：Applied biochemistry and biotechnology

authors： Liu JZ,Xu W,Chistoserdov A,Bajpai RK

更新日期：2016-07-01 00:00:00

abstract：:The changes in total phenolics, flavonoids, tannins, valerenic acid, and antioxidant activity were assessed in 25 populations of Valeriana jatamansi sampled from 1200 to 2775 m asl and four habitat types of Uttarakhand, West Himalaya. Significant (p < 0.05) variations in total phenolics, flavonoids, valerenic acid, an...

journal_title：Applied biochemistry and biotechnology

authors： Jugran AK,Bahukhandi A,Dhyani P,Bhatt ID,Rawal RS,Nandi SK

更新日期：2016-07-01 00:00:00

abstract：:Pectinases catalyze the degradation of pectic substances and are used in several processes, mainly in food and textile industries. In this study, a biomimetic matrix of alginate/gelatin/calcium oxalate (AGOCa) was synthesized for the in situ immobilization via encapsulation of crude pectinase from Aspergillus niger AT...

journal_title：Applied biochemistry and biotechnology

authors： Bustamante-Vargas CE,de Oliveira D,Valduga E,Venquiaruto LD,Paroul N,Backes GT,Dallago RM

更新日期：2016-07-01 00:00:00

abstract：:RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not ...

journal_title：Applied biochemistry and biotechnology

authors： Nübel C,Appel B,Hospach I,Mai M,Krasteva N,Nelles G,Petruschka L,Müller S

更新日期：2016-06-01 00:00:00

abstract：:Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constit...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Mariani M,de Caraffa VB,Gambotti C,Vincenti S,Desjobert JM,Muselli A,Costa J,Berti L,Maury J

更新日期：2016-06-01 00:00:00

abstract：:Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 ± 0.42 % in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 ± 1.4 %. Antioxidative ...

journal_title：Applied biochemistry and biotechnology

authors： Pawar R,Mohandass C,Dastager SG,Kolekar YM,Malwankar R

更新日期：2016-04-01 00:00:00

abstract：:Electrokinetic-microbial remediation (EMR) has emerged as a promising option for the removal of polycyclic aromatic hydrocarbons (PAHs) from contaminated soils. The aim of this study was to enhance degradation of phenanthrene (Phe)-contaminated soils using EMR combined with biosurfactants. The electrokinetic (EK) reme...

journal_title：Applied biochemistry and biotechnology

authors： Lin W,Guo C,Zhang H,Liang X,Wei Y,Lu G,Dang Z

更新日期：2016-04-01 00:00:00

abstract：:The thermal stability of most nitrile hydratases (NHase) is poor, which has been enhanced to some extent by molecular modifications in several specific regions of the C-terminal domain (C-domain) of β subunit of NHase. Since the C-domain could be present as a naturally separate domain in a few NHases, the whole C-doma...

journal_title：Applied biochemistry and biotechnology

authors： Sun W,Zhu L,Chen X,Wu L,Zhou Z,Liu Y

更新日期：2016-04-01 00:00:00

abstract：:The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y,...

journal_title：Applied biochemistry and biotechnology

authors： Wang M,Yuan W,Hale A

更新日期：2016-03-01 00:00:00

abstract：:UDPG pyrophosphatase (UGPase) plays an important role in carbohydrate metabolism, catalyzing a reversible production of uridine diphosphate glucose (UDPG) and pyrophosphate (PPi) from Glc-1-P and UTP. UGPase gene from Aureobasidium pullulans NRRL Y-12974 was cloned, overexpressed in Escherichia coli. The recombinant U...

journal_title：Applied biochemistry and biotechnology

authors： Li H,Zhang Y,Gao Y,Lan Y,Yin X,Huang L

更新日期：2016-03-01 00:00:00

abstract：:Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the ...

journal_title：Applied biochemistry and biotechnology

authors： Denisova MN,Makarova EI,Pavlov IN,Budaeva VV,Sakovich GV

更新日期：2016-03-01 00:00:00

abstract：:Rapid, accurate, and easy identification of pathogenic agents has always been important in medicine, veterinary, and agriculture. The brown spot infection is among the most common diseases in tangerine caused by Alternaria alternata. Due to the existence of seven various pathotypes of A. alternata species, it is chall...

journal_title：Applied biochemistry and biotechnology

authors： Moghimi H,Moradi A,Hamedi J,Basiri M

更新日期：2016-03-01 00:00:00

abstract：:The present study was undertaken to evaluate the effect of initial temperature treatment on phytochemical and antioxidant potential of commercially important plant Azadirachta indica A. Juss. The leaves were differentially treated after harvest at temperatures 4, -20, and 110 °C and at room temperature, separately. It...

journal_title：Applied biochemistry and biotechnology

authors： Vats S

更新日期：2016-02-01 00:00:00

abstract：:Aroma volatiles in Basmati-370, Ambemohar-157 (non-basmati scented), and IR-64 (non-scented) rice cultivars were qualitatively and quantitatively analyzed at vegetative and maturity stages to study their differential accumulation using headspace solid-phase microextraction, followed by gas chromatography mass spectrom...

journal_title：Applied biochemistry and biotechnology

authors： Hinge V,Patil H,Nadaf A

更新日期：2016-02-01 00:00:00

abstract：:Adenosine monophosphate-activated protein kinase (AMPK) is viewed as a privileged therapeutic target for several diseases such as cancer, diabetes, inflammation, obesity, etc. In addition, AMPK has entered the limelight of current drug discovery with its evolution as a key metabolic regulator. AMPK also plays a key ro...

journal_title：Applied biochemistry and biotechnology

authors： Ramesh M,Vepuri SB,Oosthuizen F,Soliman ME

更新日期：2016-02-01 00:00:00